![]() Roche There is no buffer available in which both enzymes exhibit 100 activity As these two enzymes are not compatible, Roche recommends to perform a sequential digestion to get the job done. each enzyme works at regular 1-fold concentration to obtain 100 cleavage. To view the entire NEB catalog, visit their website here. KpnI-HF/NotI-HF both exhibit 100 activities in NEBuffer 4, i.e. ![]() NEB has introduced a line of High-Fidelity (HF®) enzymes that provide added flexibility to reaction setup. High Fidelity (HF) Restriction Enzymes have 100 activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. In general, we recommend 510 units of enzyme per µg DNA, and 1020 units for genomic DNA in a 1 hour digest. A high fidelity restriction endonuclease that recognizes the sequence GCGGCCGC. Web pricing is applicable only to orders placed online at High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer. Follow with a quick ('touch') spin-down in a microcentrifuge. HF enzymes also exhibit dramatically reduced star activity. HF 386, 642, Tr 341, Pho 83 novit E, 277, ( A 545 WILAM. High Fidelity (HF) Restriction Enzymes have 100 activity in CutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. The items we carry in stock are listed in the table below as well as in the NEB UMaine Freezer Program website. On-line ordering is for Canadian customers only. An E.coli strain that carries the cloned and modified NotI gene from Nocardia otitidis-caviarum (ATCC 14630). NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).The University of Maine Freezer Program receives a 15% discount on all products available in the New England Biolabs (NEB) catalog. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. HF enzymes also exhibit dramatically reduced star activity. While most routine restriction digests are incubated for one hour or less, there are certain applications that require the addition of less than 1 unit/µg of DNA and increasing the reaction time beyond one hour. High Fidelity (HF) Restriction Enzymes have 100 activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. Restriction endonucleases vary with respect to their ability to maintain activity in a reaction over an extended period of time. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some. We are excited to announce that all reaction buffers are now BSA-free. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. NotI has a High Fidelity version NotI-HF ® ( NEB R3189 ). PvuI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10128368. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. ![]() provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. BamHI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10133983. Thermo Scientific NotI restriction enzyme recognizes GCGGCCGC sites and cuts best at 37☌ in O buffer (Isoschizomers: CciNI).
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